Exploring phenotypic overlap across schizotypy and autism spectrum conditions in American and Chinese young adults.

Competing theories have been proposed to explain the considerable overlap in social-cognitive features and risk factors across schizotypy and autism spectrum conditions (ASCs). Six previous factor analyses have been reported in the literature, yet all have major limitations; evidence for the clear superiority of any of the competing theories is insufficient and warrants further investigation. The primary aim of the present research was to identify dimensions that cut across schizotypy and ASCs while addressing limitations of past research. Data were collected from three independent samples (n = 1006, 544, and 2469) in the U.S. and China using the Autism-Spectrum Quotient, the Schizotypal Personality Questionnaire, and the Wisconsin Schizotypy Scales. Exploratory factor analyses in Sample 1 identified an interpretable three-factor structure, which was replicated in Samples 2 and 3 using confirmatory factor analyses. We found consistent evidence for three dimensions (Aberrant Salience, Asociality, and Concrete Thinking) underlying schizotypy and ASCs. This three-dimension model is consistent with a common vulnerability model of schizotypy and ASCs. Implications of these findings for the schizotypy and ASCs literature are discussed.

Developmental pathway for first onset of depressive disorders in females: from adolescence to emerging adulthood.

BACKGROUND: Although risk markers for depressive disorders (DD) are dynamic, especially during adolescence, few studies have examined how change in risk levels during adolescence predict DD onset during transition to adulthood. We compared two competing hypotheses of the dynamic effects of risk. The risk escalation hypothesis posits that worsening of risk predicts DD onset beyond risk level. The chronic risk hypothesis posits that persistently elevated risk level, rather than risk change, predicts DD onset. METHODS: Our sample included 393 girls (baseline age 13.5-15.5 years) from the adolescent development of emotions and personality traits project. Participants underwent five diagnostic interviews and assessments of risk markers for DD at 9-month intervals and were re-interviewed at a 6-year follow-up. We focused on 17 well-established risk markers. For each risk marker, we examined the prospective effects of risk level and change on first DD onset at wave six, estimated by growth curve modeling using data from the first five waves. RESULTS: For 13 of the 17 depression risk markers, elevated levels of risk during adolescence, but not change in risk, predicted first DD onset during transition to adulthood, supporting the chronic risk hypothesis. Minimal evidence was found for the risk escalation hypothesis. CONCLUSIONS: Participants who had a first DD onset during transition to adulthood have exhibited elevated levels of risk throughout adolescence. Researchers and practitioners should administer multiple assessments and focus on persistently elevated levels of risk to identify individuals who are most likely to develop DD and to provide targeted DD prevention.

Quantitative transport and immobilization of cadmium in saturated-unsaturated soils with the combined application of biochar and organic fertilizer.

In this study, cadmium (Cd) transport and immobilization on passivators (biochar, organic fertilizer) and soils under saturated-unsaturated conditions were independently analyzed. The results showed that the Cd adsorption capacities of biochar and organic fertilizer were comparable in acidic soils. But in alkaline soils, the Cd adsorption capacity of organic fertilizer was significantly larger than that of biochar. In acidic soils, passivators effectively immobilized Cd, and the total net effects were in the order: combination (44.05-58.13%) > 3% biochar (31.96-46.88%) > 3% organic fertilizer (28.78-41.82%). In alkaline soils, all treatments had negative effects on Cd immobilization. For acidic soils, the immobilization of Cd was mainly attributed to the passivators, and the positive contribution percentages of relatively stable Cd increase by passivators were 81.05-100%, while those by soils were 0-18.95%. For alkaline soils, after the treatments of passivators, although a considerable amount of Cd was immobilized inside the passivator, Cd was activated more inside the soil. Therefore, it is noteworthy that soil conditions must be fully considered when applying biochar and organic fertilizers for Cd remediation.

Dynamic risk for first onset of depressive disorders in adolescence: does change matter?

BACKGROUND: Risk factors for depressive disorders (DD) change substantially over time, but the prognostic value of these changes remains unclear. Two basic types of dynamic effects are possible. The 'Risk Escalation hypothesis' posits that worsening of risk levels predicts DD onset above average level of risk factors. Alternatively, the 'Chronic Risk hypothesis' posits that the average level rather than change predicts first-onset DD. METHODS: We utilized data from the ADEPT project, a cohort of 496 girls (baseline age 13.5-15.5 years) from the community followed for 3 years. Participants underwent five waves of assessments for risk factors and diagnostic interviews for DD. For illustration purposes, we selected 16 well-established dynamic risk factors for adolescent depression, such as depressive and anxiety symptoms, personality traits, clinical traits, and social risk factors. We conducted Cox regression analyses with time-varying covariates to predict first DD onset. RESULTS: Consistently elevated risk factors (i.e. the mean of multiple waves), but not recent escalation, predicted first-onset DD, consistent with the Chronic Risk hypothesis. This hypothesis was supported across all 16 risk factors. CONCLUSIONS: Across a range of risk factors, girls who had first-onset DD generally did not experience a sharp increase in risk level shortly before the onset of disorder; rather, for years before onset, they exhibited elevated levels of risk. Our findings suggest that chronicity of risk should be a particular focus in screening high-risk populations to prevent the onset of DDs. In particular, regular monitoring of risk factors in school settings is highly informative.

Etiologic identification and drug susceptibility analysis of a Citrobacter freundii food poisoning event / 中国热带医学

@#Abstract: Objective　In order to provide reference for emergency treatment of a sudden food poisoning incident, pathogen detection and drug resistance analysis were carried out. Methods　Diarrheal stool and surplus food samples were detected by GB 4789 and the isolates were identified by VITEK2 and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), at the same time, the bacterial drug sensitivity test was carried out by using the method of microbroth dilution, and the isolates from different sources were molecularly classified by pulsed field gel electrophoresis (PFGE), and the correlation between the strains was analyzed by BioNumerics software. Results　Totaly 13 leftovers and 3 diarrhea patients were isolated and identified, The total number of colonies and coliforms in 7 leftovers samples all exceeded the standard, and Citrobacter freundii was detected in 5 leftovers and 2 stools. The results of drug sensitivity test showed that seven strains of Citrobacter freundii were sensitive to ciprofloxacin, tetracycline, chloramphenicol, gentamicin, amikacin, cefotaxime and meropenem, but completely resistant to ampicillin, and there was no multiple drug resistance. The results of pulsed field gel electrophoresis (PFGE) showed that 7 strains of Citrobacter freundii had the same PFGE bands and 100% homology, showing the same clone. Conclusions　This food poisoning incident was caused by Citrobacter freundii. The pathogen of food poisoning can be quickly and accurately determined by MALDI-TOF MS, which is beneficial to the early diagnosis and treatment of infectious diseases. It is suggested to strengthen the corresponding management, improve food safety awareness and prevent similar incidents.

Testing a theory-driven factor structure of the autism-spectrum quotient.

The Autism-Spectrum Quotient (AQ) is a popular instrument used to assess the degree to which individuals exhibit features of autism spectrum conditions (ASC). The current study aimed to develop a theory-driven factor structure of the AQ that would fit as well across samples as the 12 previously proposed factor structures, all of which, except for the original Baron-Cohen model, had been developed on the basis of exploratory factor analysis (EFA) or principal component analysis. We first proposed a six-factor solution: (1) social anhedonia; (2) interest in details/patterns; (3) imagination ability; (4) desire for predictability/routine; (5) social cognition; and (6) social discourse convention. We tested the six-factor structure and made final item selections (27 items) with EFA using data from college students (n = 503). Then, we empirically tested alternative factor structure models in three other independent samples (ns = 503; 1263; 1641) using confirmatory factor analysis. Results indicated that our model fit as well, if not better, than all of the other models across samples, regardless of parameter estimation methods and software packages. Overall, the theory-driven replicable six-factor structure that we report holds the potential to be used to measure the six domains of features that we identified in the AQ. LAY SUMMARY: Questionnaire measures of autism spectrum conditions have typically been used to measure approximately four broad dimensions. Our study suggests that the Autism-Spectrum Quotient can be used to measure six more narrowly defined dimensions: social anhedonia, interest in details/patterns, imagination ability, desire for predictability/routine, social cognition, and social discourse convention. Additional work is needed to develop measures of a much wider variety of autism spectrum features.

Cadmium long-term immobilization by biochar and potential risks in soils with different pH under combined aging.

Cd long-term immobilization by biochar and potential risk in soils with different pH were quantified under a combined artificial aging, which simulated five years of aging in the field based on local climate. Two biochars (original and KMnO4-modified) and five soils with different pH were tested, and an improved three-layer mesh method was employed in this study. Five aging cycles were carried out (Cycle 1-Cycle 5), and each aging cycle quantitatively simulated 1 year of natural aging. As the aging time increased, Cd leaching loss in all soils gradually increased from Cycle 1 to Cycle 5; for relatively stable Cd fraction, it decreased firstly and then stabilized in acidic and neutral soils (S1-S4), while it decreased firstly and then increased in alkaline soil (S5). Biochars significantly promoted Cd immobilization in strongly acidic soil (S1) by increasing relatively stable fractions and decreasing leaching loss. For weakly acidic and neutral soils (S2-S4), although biochars still had positive effects, the immobilization effects were weakened to certain extents compared with S1. The percentage of Cd leaching loss decreased by 19.12% in strongly acidic soil (S1) and by 1.12-11.35% in weakly acidic and neutral soils (S2-S4) after modified biochar treatment. For alkaline soil (S5), the application of biochars had negative effects on Cd immobilization by decreasing relatively stable fractions and increasing leaching loss, and posed risks to the environment. For strongly acidic soil (S1) and weakly acidic and neutral soils (S2-S4), the percentages of relatively stable fractions increased from 6.09-19.93% to 24.98-36.70% after modified biochar treatment. However, for alkaline soil, the percentage of relatively stable fractions decreased from 55.27% to 53.93% after biochar treatment. The more acidic the soil, the more effective the Cd immobilization by biochar. Biochars with high pH level are not suitable for the remediation of alkaline Cd contaminated soil.

Effects of competitive adsorption with Ni(II) and Cu(II) on the adsorption of Cd(II) by modified biochar co-aged with acidic soil.

To investigate the effects of competitive adsorption with Ni(II) and Cu(II) on the adsorption of Cd(II) by modified biochar co-aged with acidic soil, four biochars were employed in this study, namely original biochar, KMnO4-modified biochar and two aged biochars which co-aged with an acidic soil using above biochars under freeze-thaw cycling and dry-wet cycling for 54 days simulating 6 years of natural aging. The results showed that biochar adsorption capacities of three heavy metal ions were in the order of Cd(II) > Cu(II) > Ni(II) in the single system while Cu(II) > Cd(II) > Ni(II) in binary and ternary systems. Modification improved biochar adsorption capacity of Cd(II), but competitive adsorption with Ni(II) and Cu(II) weakened the improvement of modification on adsorption performance of modified biochar in binary and ternary systems. The QMBC/QBC of Cd(II) (QMBC and QBC are the adsorption capacities of heavy metals by modified and original biochars) decreased from 231.57% (single system) to 216.67%∼219.41% (binary system) and further decreased to 207.74% (ternary system). Co-aging with soil weakened the adsorption capacities of biochars for Cd(II), even worse, competition aggravated this negative effect of co-aging. The QAMBC/QMBC of Cd(II) (QAMBC is the adsorption capacities of heavy metals by aged modified biochar) decreased from 65.41% (single system) to 14.43%∼19.46% (binary and ternary systems). Therefore, the impact of competition should be fully considered when evaluating Cd long-term remediation effects of modified biochar in Cd polluted soils accompanied with other heavy metals.

The Association of Obsessive-Compulsive Disorder, Anxiety Disorders, and Posttraumatic Stress Disorder with Impairment Related to Eating Pathology.

Prior work has shown a number of similarities between obsessive-compulsive disorder (OCD) and eating disorders such as perfectionism and depressive symptoms. However, distress and impairment due to eating pathology are also highly comorbid with other disorders, which brings into question whether the relationship with eating pathology is unique to OCD. The aims of the current study were 1) to test perfectionism and depression as mediators of the relationship between OCD and eating pathology, and 2) to determine whether OCD is related to greater distress/impairment regarding eating habits, exercising, or feelings about eating, shape, or weight above and beyond other disorders. Symptoms were assessed in 329 treatment-seeking patients in a secondary analysis of a clinical battery. The results showed that depressive symptoms and perfectionism were found to mediate the relationship between OCD and eating pathology. Additionally, a regression analysis showed that OCD, social anxiety disorder, and panic disorder symptoms were associated with eating pathology to a greater extent than other disorders. These results suggest that distress and impairment related to eating habits, exercising, or feelings about eating, shape, or weight are not unique to OCD and that depression and perfectionism may, in part, explain the association between OCD and eating pathology.

Under-documentation of psychiatric diagnoses among persons living with HIV in electronic medical records.

Persons living with HIV (PLWH) are significantly more likely to meet criteria for a psychiatric disorder compared to the general population. To our knowledge there are no prior studies that report rates of psychiatric diagnosis documentation in electronic medical records (EMRs) of PLWH. The goal for this study was to report the rates of a variety of psychiatric diagnoses among PLWH in electronic medical records. Participants (n = 2,336) were enrolled in the Center for AIDS Research (CFAR) Longitudinal Database study at the University of Pennsylvania, Philadelphia, PA. Diagnostic codes were extracted from the EMR for depressive disorders, alcohol and substance use disorders, PTSD, sleep disorders, and adjustment disorders and were compared to rates from national epidemiological studies. Rates of Major Depressive Disorder in the EMR were comparable to prior reports on HIV-infected samples. In contrast, rates of PTSD, substance use disorders, alcohol use disorders, adjustment disorders and insomnia from the EMR were all markedly lower compared to national estimates for HIV-infected samples. While clinicians appropriately documented evidence of Major Depressive Disorder, other psychiatric comorbidities were largely overlooked. These findings suggest a potential bias in how clinicians either detect or document psychiatric disorders in PLWH.

The unique contribution of perfectionistic cognitions to anxiety disorder symptoms in a treatment-seeking sample.

Perfectionistic cognitions are thinking patterns that reflect excessive striving and are associated with emotional disorders in nonclinical samples. Despite literature connecting trait perfectionism with psychological disorders, much remains unknown about how perfectionistic cognitions relate to anxiety disorder symptoms in clinical populations. This is the first study to our knowledge that investigates how symptoms of anxiety and related symptoms are influenced by the frequency of perfectionistic cognitions when controlling for well documented correlates of anxiety. Perfectionistic cognitions, depressive symptoms, emotion regulation, anxiety sensitivity, and anxiety symptom severity were assessed prior to starting treatment in 356 treatment-seeking patients diagnosed with an anxiety or anxiety-related disorder at a specialty anxiety clinic. Perfectionistic cognitions were significantly correlated with all anxiety symptom measures as well as measures of depression, emotion regulation and anxiety sensitivity (range of rs =.22-.68). Hierarchical regression analyses revealed that when controlling for depressive symptoms, anxiety sensitivity, and emotion regulation, perfectionistic cognitions significantly and uniquely contribute to the variance of GAD (p <.01) and PTSD (p <.05) symptoms but not other anxiety-related symptoms (all ps >.05). Regardless of specific diagnoses, treatment-seeking individuals reporting frequent perfectionistic thoughts are more likely to report more severe symptoms of PTSD and GAD.

Suicide risk among persons living with HIV.

Persons living with HIV (PLWH) are significantly more likely to die by suicide compared to the general population. This is the first study to examine the impact of posttraumatic stress disorder (PTSD), major depressive disorder (MDD), insomnia, and substance use disorders on suicidal ideation/behavior among PLWH using electronic medical record (EMR) data. We also evaluated the mutual influence of interactions between PTSD and substance use disorders on suicide risk, consistent with the substance abuse, violence exposure, and HIV/AIDS "SAVA" syndemic model. Participants (n = 2336) were HIV+ individuals recruited through the Center for AIDS Research (CFAR). Participants provided informed consent for extraction of their EMR. As hypothesized, univariate analyses revealed that PTSD, MDD, insomnia, alcohol and other substance use disorders (cocaine abuse and cocaine dependence, opioid abuse and dependence, cannabis abuse, other psychoactive substance abuse and dependence, and polysubstance use disorder) were each positively associated with suicidal ideation/behavior. Also as hypothesized, a multivariable analysis found that alcohol and cocaine dependence, MDD, and PTSD were significant predictors of suicidal ideation/behavior. Contrary to hypotheses, none of the interactions between PTSD and substance use disorders were significantly associated with suicidal ideation/behavior.

Trauma-related cognitions predict treatment response in smokers with PTSD: Evidence from cross-lagged panel analyses.

OBJECTIVE: Compared to smokers without posttraumatic stress disorders (PTSD), smokers with PTSD smoke more heavily and are less successful in quitting smoking. However, limited research has examined the cognitive pathways underlying this heightened comorbidity. The current study is the first to simultaneously model the cross-sectional and lagged relationships between trauma-related cognitions and cigarette smoking, as well as between trauma-related cognitions and PTSD severity, in smokers with comorbid PTSD receiving treatment. METHOD: Participants (n = 142) were seeking treatment for smoking cessation and PTSD as part of a randomized controlled trial of varenicline and smoking cessation counseling with or without adjunctive Prolonged Exposure (varenicline + PE vs. varenicline only) (Foa et al., 2017). Data were available for both baseline and end-of-treatment assessments of trauma cognitions severity of cigarette smoking and PTSD symptoms. We conducted both cross-sectional and lagged analysis to simultaneously examine the bidirectional relationship from trauma cognitions and 1) cigarette smoking and 2) PTSD symptoms. RESULTS: Trauma cognitions (specifically, negative beliefs about the self and the world) were significantly associated with cigarette/day at the end of treatment for participants who received varenicline only. However, baseline trauma cognitions did not predict post-treatment cigarettes/day. Baseline trauma cognitions (specifically negative beliefs about the self and world) were associated with PTSD severity at both baseline and end of treatment; furthermore, these negative cognitions at baseline positively and prospectively predicted end-of-treatment PTSD severity, but not vice versa. Wald tests revealed that there were no treatment effects on these cross-lagged relationships. Conclusions These findings provide novel empirical support for the importance of addressing trauma-related cognitions in the smoking cessation treatment efforts for patients with comorbid PTSD and cigarette smoking.

Machine Learning Algorithms in Suicide Prevention: Clinician Interpretations as Barriers to Implementation.

OBJECTIVE: Machine learning algorithms in electronic medical records can classify patients by suicide risk, but no research has explored clinicians' perceptions of suicide risk flags generated by these algorithms, which may affect algorithm implementation. The objective of this study was to evaluate clinician perceptions of suicide risk flags. METHODS: Participants (n = 139; 68 with complete data) were mental health clinicians recruited to complete online surveys from October 2018 to April 2019. RESULTS: Most participants preferred to know which features resulted in a patient receiving a suicide flag (94.12%) and reported that knowing those features would influence their treatment (88.24%). Clinicians were more likely to report that some algorithm features (increased thoughts of suicide) would alter their clinical decisions more than others (age, physical health conditions; χ² = 270.84, P < .001). Clinicians were more likely to report that they would create a safety/crisis response plan in response to a suicide risk flag compared to other interventions (χ² = 227.02, P < .001), and 21% reported that they would complete a no-suicide contract following a suicide risk flag. CONCLUSIONS: Clinicians overwhelmingly reported that suicide risk flags in electronic medical records would alter their clinical decision making. However, clinicians' likelihood of acting in response to a suicide risk flag was tied to which features were highlighted rather than the presence of the risk flag alone. Thus, the utility of a suicide risk algorithm will be reduced if clinical features underlying the algorithm are hidden from clinicians or if clinicians do not view the clinical features as intuitively meaningful predictors of suicide risk.

The Effect of Shame on Patients With Social Anxiety Disorder in Internet-Based Cognitive Behavioral Therapy: Randomized Controlled Trial.

BACKGROUND: Prior research has demonstrated the efficacy of internet-based cognitive behavioral therapy (ICBT) for social anxiety disorder (SAD). However, it is unclear how shame influences the efficacy of this treatment. OBJECTIVE: This study aimed to investigate the role shame played in the ICBT treatment process for participants with SAD. METHODS: A total of 104 Chinese participants (73 females; age: mean 24.92, SD 4.59 years) were randomly assigned to self-help ICBT, guided ICBT, or wait list control groups. For the guided ICBT group, half of the participants were assigned to the group at a time due to resource constraints. This led to a time difference among the three groups. Participants were assessed before and immediately after the intervention using the Social Interaction Anxiety Scale (SIAS), Social Phobia Scale (SPS), and Experience of Shame Scale (ESS). RESULTS: Participants' social anxiety symptoms (self-help: differences between pre- and posttreatment SIAS=-12.71; Cohen d=1.01; 95% CI 9.08 to 16.32; P<.001 and differences between pre- and posttreatment SPS=11.13; Cohen d=0.89; 95% CI 6.98 to 15.28; P<.001; guided: SIAS=19.45; Cohen d=1.20; 95% CI 14.67 to 24.24; P<.001 and SPS=13.45; Cohen d=0.96; 95% CI 8.26 to 18.64; P<.001) and shame proneness (self-help: differences between pre- and posttreatment ESS=7.34; Cohen d=0.75; 95% CI 3.99 to 10.69; P<.001 and guided: differences between pre- and posttreatment ESS=9.97; Cohen d=0.88; 95% CI 5.36 to 14.57; P<.001) in both the self-help and guided ICBT groups reduced significantly after treatment, with no significant differences between the two intervention groups. Across all the ICBT sessions, the only significant predictors of reductions in shame proneness were the average number of words participants wrote in the exposure module (ß=.222; SE 0.175; t96=2.317; P=.02) and gender (ß=-.33; SE 0.002; t77=-3.13; P=.002). We also found a mediation effect, wherein reductions in shame fully mediated the relationship between the average number of words participants wrote in the exposure module and reductions in social anxiety symptoms (SIAS: ß=-.0049; SE 0.0016; 95% CI -0.0085 to -0.0019 and SPS: ß=-.0039; SE 0.0015; 95% CI -0.0075 to -0.0012). CONCLUSIONS: The findings of this study suggest that participants' engagement in the exposure module in ICBT alleviates social anxiety symptoms by reducing the levels of shame proneness. Our study provides a new perspective for understanding the role of shame in the treatment of social anxiety. The possible mechanisms of the mediation effect and clinical implications are discussed. TRIAL REGISTRATION: Chinese Clinical Trial Registry ChiCTR1900021952; http://www.chictr.org.cn/showproj.aspx?proj=36977.

Overexpression of CmSOS1 confers waterlogging tolerance in Chrysanthemum.

The Na+ /H+ antiporter SOS1 enhances the salinity tolerance of a number of plant species, but its involvement in the response to hypoxia is less well known. We presented chrysanthemum homologs CmSOS1 and CmRCD1 coordinately mediate waterlogging tolerance by maintaining membrane integrity and minimizing the level of reactive oxygen species.

Shame aversion and maladaptive eating-related attitudes and behaviors.

Consistent with affect regulation models of eating-related psychopathology, prior research indicates that trait-like shame and state self-conscious emotion are associated with maladaptive eating-related attitudes/behaviors. The present investigation extended past research on shame and maladaptive eating-related attitudes/behaviors by examining the role of shame aversion - the perception of shame as an especially unwanted and painful emotion - in these attitudes/behaviors over and above shame-proneness, general distress, and experiential avoidance. In 488 female undergraduates, shame aversion was positively associated with dieting, self-perceptions of body shape, awareness of food content, and food preoccupation even after taking into account other possible explanatory variables. Additionally, shame aversion moderated shame-proneness' associations with dieting, awareness of food content, and food preoccupation, such that shame-proneness was positively associated with these attitudes/behaviors only when shame aversion was high. Future directions for research and clinical implications of the present findings are discussed.

[Terminal restriction fragment length polymorphism analysis of endolithic bacteria community at special habitats in Xinjiang].

OBJECTIVE: In order to investigate the composition and diversity of endolithic bacteria at special habitats in Xinjiang. METHODS: Five rock samples were collected, including Wusu's granite (sample 1), Glacier No. 1, and Mulei's metamorphic rock (sample 2, sample 3), Yumin and Tokesun's Rock varnish (sample 4, sample 5). Endolithic bacterial community composition and diversity were analyzed by the method of Terminal Restriction Fragment Length Polymorphism. RESULTS: Differences in diversity indexes among samples were not apparent. Clustering analysis suggested that similarity coefficient was higher in same rock type, sample 2 and sample 3 were grouped together, then sample 1 clustered with them, and sample 4 and sample 5 were classified together. All samples harbored these phyla such as Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes. Acidobacteria and Planctomycetes existed in sample 1 and sample 2, respectively; Sample 5 was dominated by Actinobacteria, while other samples were dominated by Proteobacteria. CONCLUSION: The endolithic bacterial composition of same rock type collected at various habitats was different. Meanwhile, a diversity of novel species and lineages maybe existed in rocks.